Biuret Protein Colorimetric Assay Kit

Biuret Protein Colorimetric Assay Kit

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A A-R Mohamed et al found that selenium nanoparticle can alleviate cardio-hepatic damage in type II diabetes. Total protein (TP) level in rat serum was determined using biuret protein colorimetric assay kit (E-BC-K165-M).

Table. Effect of different treatments (metformin at a dose of (500 mg/kg) and /or CTS-Se-NPs (2 mg Se/kg per day) in HFD/STZ diabetic rats on glucose level, insulin, lipid profile and hepatic oxidative stress.

TP level was significantly induced in HFD/STZ treated group.

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.58-100 g/L).

The recommended dilution factor for different samples is as follows (for reference only):

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Detection Principle

Any compound that contains two -CONH2 in the molecule can react with alkaline copper solution to form a purple complex, which is known as the biuret reaction. Many peptide bonds (-CONH-) in protein molecules can perform this reaction, and the color degree of all kinds of proteins are essentially the same.

Kit Components & Storage

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 98%.

Sensitivity

The analytical sensitivity of the assay is 0.58 g/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.

Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only: