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Bradford Protein Colorimetric Assay Kit
SKU: E-BC-K168-S-500
Bradford Protein Colorimetric Assay Kit
| SKU # | E-BC-K168-S |
| Detection Instrument | Spectrophotometer (595 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | TP |
| Sample Type | Serum, plasma, animal tissue |
| Sensitivity | 0.026 mg/mL |
| Detection Range | 0.026-1.2 mg/mL |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 50 min |
| Precision | Average inter-assay CV: 2.300% | Average intra-assay CV: 1.800% |
| Other instruments required | Micropipettor, Vortex mixer |
| Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
| Storage | Reagent 1: 2-8℃, Reagent 2: RT |
| Valid period | 12 months |
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.026-1.2 mg/mL).
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 40-60 |
| 10% Mouse liver tissue homogenization | 8-12 |
| Rat serum | 40-60 |
Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).
Detection Principle
Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.
Kit Components & Storage
| Item | Component | Specification | Storage |
| Reagent 1 | Chromogenic Agent Stock Solution | 35 mL × 2 vials | 2-8℃, shading light, 12 months |
| Reagent 2 | 0.563 mg BSA Standard | 0.563 mg × 2 vials | RT, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other.