Accuris Hot Start Taq Polymerase (Supplied Separate from Buffer or with 2X Master Mix)

Accuris Hot Start Taq Polymerase (Supplied Separate from Buffer or with 2X Master Mix)

Engineered for controlled polymerase activity, Accuris Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.

When samples are ready, the reaction mixture is heated to 95°C to denature the antibody and initiate the reaction. Similar to the standard Accuris Taq, Hot Start Taq is provided with a 5X buffer, or in a ready to use 2X Master Mix. The Master Mix can be ordered with or without an incorporated red gel loading dye. The Red Dye Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel. The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

• Exceptional sensitivity for low copy PCR.
• Ideal for multiplex PCR and amplification of GC-rich DNA.
• Same enhanced features as Accuris Taq Polymerase.
• Buffer optimized for fast cycling and reproducibility.
• Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer (dNTPs not included).

The polymerase is supplied with a 5X buffer containing MgCl₂ and proprietary mix of enhancers (dNTP’s not included). For convenience, Accuris Taq is also available in a ready to use 2X Master Mix – just add primers and template DNA. Our “Red Dye” Master Mix incorporates a red loading dye that allows amplified samples to be loaded directly on an agarose gel. The red color aids in visualization during pipetting, and higher density of the buffer ensures that the samples will drop into the gel wells.

Specifications

MSE supplies is an authorized distributor of Accuris products and accessories.