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Formate Colorimetric Assay Kit
SKU: E-BC-K1100-M-96
Formate Colorimetric Assay Kit
| SKU # | E-BC-K1100-M |
| Detection Instrument | Microplate reader (450 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Sample Type | Serum, plasma, animal tissue |
| Sensitivity | 8.20 μmol/L |
| Detection Range | 8.20-800 μmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 70 min |
| Precision | Average inter-assay CV: 3.900% | Average intra-assay CV: 3.300% |
| Other instruments required | Pipettor, Water bath, Centrifuge |
| Storage | -20℃ |
| Valid period | 12 months |
Dilution of Sample
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (8.20-800 μmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| Human serum | 1 |
| Dog serum | 1 |
| Mouse serum | 1 |
| Horse serum | 1 |
| Porcine serum | 1 |
| Rat plasma | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
Note: The diluent is reagent 1.
Detection Principle
Formic acid is the simplest carboxylic acid, whose chemical formula is CH2O2. It is often used as an antimicrobial/preservative in livestock feed. Formic acid can block some of the decay processes in feed, making the nutritional value of feed maintain longer. Under normal circumstances, the physiological concentration of formic acid is low and easy to metabolize, but in the case of methanol poisoning, the concentration can reach 5 mmoL/L. Similarly, long-term exposure to excessive levels of formaldehyde can also increase the content of formic acid in blood and urine.
Kit Components & Storage
| Item | Component | Size (96 T) | Storage |
| Reagent 1 | Extracting Solution | 60 mL × 2 vials | -20℃, 12 months |
| Reagent 2 | Substrate A | Powder × 2 vials | -20℃, 12 months, shading light |
| Reagent 3 | Substrate B | Powder × 1 vial | -20℃, 12 months, shading light |
| Reagent 4 | Chromogenic Agent | 1.5 mL × 2 vials | -20℃, 12 months, shading light |
| Reagent 5 | 10 mmol/L Standard | 1.0 mL × 1 vial | -20℃, 12 months |
| Microplate | 96 wells | No requirement | |
| Plate Sealer | 2 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (μmol/L) | 16.50 | 135.00 | 264.00 |
| %CV | 3.6 | 3.2 | 3.1 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (μmol/L) | 16.50 | 135.00 | 264.00 |
| %CV | 4.1 | 3.8 | 3.8 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 100%.
| Standard 1 | Standard 2 | Standard 3 | |
| Expected Conc. (μmol/L) | 125 | 362 | 554 |
| Observed Conc. (μmol/L) | 126.3 | 358.4 | 554.0 |
| Recovery rate (%) | 101 | 99 | 100 |
Sensitivity
The analytical sensitivity of the assay is 8.2 μmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
| Concentration (mmol/L) | 0 | 100 | 200 | 300 | 400 | 500 | 600 | 800 |
| Average OD | 0.154 | 0.327 | 0.492 | 0.677 | 0.838 | 1.025 | 1.171 | 1.593 |
| Absoluted OD | 0.000 | 0.174 | 0.338 | 0.524 | 0.684 | 0.872 | 1.017 | 1.440 |