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Glycerol Colorimetric Assay Kit
SKU: E-BC-K340-M-96
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Glycerol Colorimetric Assay Kit
| SKU # | E-BC-K340-M |
| Detection Instrument | Microplate reader (500-520 nm, optimum wavelength: 510 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Sample type | Serum (plasma), animal tissue, cell |
| Sensitivity | 0.01mmol/L |
| Detection range | 0.01-1.00 mmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Assay time | 10 min |
| Precision | Average inter-assay CV: 8% | Average intra-assay CV: 4% |
| Other instruments required | Water bath |
| Other reagents required | Double distilled water, Isopropanol (AR), PBS(0.01 M，pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| 10% Mouse liver tissue homogenate | 2 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| Cell | 1 |
Note: The diluent is double distilled water. For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
Glycerol is transformed by enzyme to produce hydrogen peroxide, which is catalyzed by oxidase in the presence of 4-amino-antipyrprid and phenol to produce red quinones, the color of which is proportional to the content of glycerol.Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Working Solution | 12.5 mL × 1 vial | 25 mL × 1 vial | 2-8℃, 12 months shading light |
| Reagent 2 | 1.0 mmol/L Standard | 1 mL × 1 vial | 1 mL × 2 vials | 2-8℃, 12 months shading light |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.25 | 0.64 | 0.82 |
| %CV | 4.2 | 3.9 | 3.9 |
Inter-assay Precision
Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.25 | 0.64 | 0.82 |
| %CV | 8.2 | 7.8 | 8.0 |
Sensitivity
The analytical sensitivity of the assay is 0.01mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
|
Concentration (mmol/L) |
0.00 | 0.10 | 0.20 | 0.40 | 0.50 | 0.60 | 0.80 | 1.00 |
| Average OD | 0.072 | 0.122 | 0.175 | 0.293 | 0.340 | 0.394 | 0.505 | 0.601 |
| Absoluted OD | 0.000 | 0.050 | 0.103 | 0.221 | 0.269 | 0.323 | 0.433 | 0.530 |