Human COL10 (Collagen Type X) CLIA Kit
SKU: E-CL-H0563
Human COL10 (Collagen Type X) CLIA Kit
Detection Range | 12.50-800 pg/mL |
Sensitivity | 7.50 pg/mL |
Product Details
Properties
Assay type | Sandwich-CLIA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 12.50-800 pg/mL |
Sensitivity | 7.50 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human COL10 in samples. No significant cross-reactivity or interference between Human COL10 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 15%. |
Application | This CLIA kit applies to the in vitro quantitative determination of Human COL10 concentrations in serum, plasma and other biological fluids. |
Test Principle
Kit Components & Storage
An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Item | Specifications | Storage |
---|---|---|
Micro CLIA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃(Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Technical Data:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human COL10 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human COL10 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 43.82 | 76.72 | 344.80 | 47.71 | 77.17 | 374.85 |
Standard deviation | 4.99 | 7.84 | 28.31 | 5.46 | 8.52 | 23.65 |
CV (%) | 11.39 | 10.22 | 8.21 | 11.44 | 11.04 | 6.31 |
Recovery
The recovery of Human COL10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-102 | 93 |
EDTA plasma(n=8) | 95-112 | 102 |
Cell culture media(n=8) | 89-101 | 95 |
Assay Procedures
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1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃. |
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![]() 2.Remove the liquid. |
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![]() 3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times. |
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![]() 4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times. |
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![]() 5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃. |
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![]() 6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results. |