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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)– MSE Supplies LLC

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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

SKU: E-BC-K025-S-100

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Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method)

SKU # E-BC-K025-S
Detection Instrument Spectrophotometer(532 nm)
Detection Method Colorimetric method


Product Details

Properties

Synonyms MDA
Sample Type Serum, plasma, animal tissue
Sensitivity 0.38 nmol/mL
Detection Range 0.38-133.33 nmol/mL
Detection Method Colorimetric method
Assay type Quantitative
Assay time 90 min
Precision Average inter-assay CV: 8% | Average intra-assay CV: 4.900%
Other instruments required Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4), Glacial acetic acid (analytical reagent, acetic acid concentration ≥99.5%), Absolute ethanol
Storage 2-8℃
Valid period 12 months

 

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the formal experiment and the detection range (0.38-133.33 nmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Human serum 1
Human plasma 1
Rat serum 1
Rat plasma 1
Mouse serum 1
Mouse plasma 1
10% Rat heart tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Rat spleen tissue homogenate 1
10% Rat lung tissue homogenate 1
10% Rat kidney tissue homogenate 1
10% Rat brain tissue homogenate 1

 

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

 

Detection Principle

MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.


Kit Components & Storage

Item Component Size 1 (48 T) Size 2 (96 T) Storage
Reagent 1 Clarificant 12 mL × 1 vial 24 mL × 1 vial 2-8℃, 12 months
Reagent 2 Acid Reagent 12 mL × 1 vial 12 mL × 1 vial 2-8℃, 12 months
Reagent 3 Chromogenic Agent Powder × 1 vial Powder × 2 vials 2-8℃, 12 months
shading light
Reagent 4 10 nmol/mL Standard 5 mL × 1 vial 5 mL ×1 vial 2-8℃, 12 months

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters Sample 1 Sample 2 Sample 3
Mean (nmol/mL) 1.20 35.60 102.50
%CV 5.3 4.8 4.6


Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (nmol/mL) 1.20 35.60 102.50
%CV 7.8 8.2 8.0


Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 101%.


Sample 1 Sample 2 Sample 3
Expected Conc. (nmol/mL) 27.5 88.4 112.3
Observed Conc. (nmol/mL) 27.2 90.2 114.5
Recovery rate (%) 99 102 102


Sensitivity

The analytical sensitivity of the assay is 0.38 nmol/mL MDA. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.