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Monoamine Oxidase (MAO) Activity Assay Kit
SKU: E-BC-K008-M-96
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Monoamine Oxidase (MAO) Activity Assay Kit
| SKU # | E-BC-K008-M |
| Detection Instrument | Microplate reader(345 nm-360 nm, optimum wavelength: 355 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Synonyms | MAO |
| Sample Type | Animal tissue |
| Sensitivity | 16 U/L |
| Detection Range | 16 – 641 U/L |
| Detection Method | Colorimetric method |
| Assay Time | 40 min |
| Precision | Inter-assay CV: 6% | intra-assay CV: 3.3% |
| Storage | This product can be stored at 2~8°C for 12 months with shading light. |
| Valid period | 12 months |
Dilution Of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
The diluent is buffer solution working solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
MAO can catalyze 4-dimethylambenzylamine to produce p-dimethylaminobenzaldehyde. p-Dimethylaminobenzaldehyde has a characteristic absorption peak at 355 nm. The activity of MAO can be calculated indirectly by analyzing the production of p-dimethylaminobenzaldehyde.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Extraction Solution A | 30 mL ×1 vial | 60 mL ×1 vial | 2-8°C, 12 months |
| Reagent 2 | Extraction Solution B | 60 mL ×1 vial | 60 mL × 2 vials | 2-8°C, 12 months |
| Reagent 3 | Buffer Solution | 60 mL ×1 vial | 60 mL × 2 vials | 2-8°C, 12 months |
| Reagent 4 | Chromogenic Agent | 3 mL ×1 vial | 5 mL ×1 vial | 2-8°C, 12 months |
| UV Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three rat liver tissue samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
|
Mean (U/L) |
25.00 | 240.00 | 350.00 |
| %CV | 3.5 | 3.1 | 3.3 |
Inter-assay Precision
Three rat liver tissue samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
|
Mean (U/L) |
25.00 | 240.00 | 350.00 |
| %CV | 5.7 | 6.2 | 6.1 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 100%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (U/L) | 90 | 285 | 460 |
| Observed Conc. (U/L) | 9.27 | 305.0 | 483.0 |
| Recovery rate (%) | 103 | 107 | 105 |
Sensitivity
The analytical sensitivity of the assay is 16 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.