MSE PRO Collagenase, CLSAFC, Filtered

MSE PRO™ Collagenase, CLSAFC, Filtered

The MSE PRO™ Crude collagenase preparations contain several isoforms of two different collagenases, a mercapto protease, clostridial protease, a trypsin-like enzyme and an aminopeptidase. This combination of collagenolytic and protein hydrolytic activities effectively breaks down the intercellular matrix and is an important component of tissue dissociation. One component of the complex is a hydrolase that preferentially degrades helical regions in natural collagen, preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is usually a neutral amino acid. Crude collagenase is inhibited by metal chelators such as cysteine, EDTA or o-phenanthroline, but not by DFP. It is also inhibited by α2-macroglobulin (a large plasma glycoprotein).

The enzyme requires calcium ions for activation. The specific enzyme profile of each collagenase correlates with the organization of the cells used for the study (or the purpose for which the cells were used). And because of the correlation, we have established several types of crude collagenase: type 1, type 2, type 3, and type 4. type 1 crude collagenase has a primitive proportion of collagenase with tyrosinase, clostridial protease, and tryptic activity. type 2 contains higher relative levels of protease activity, especially clostridial protease. type 3 contains the lowest levels of secondary proteases. type 4 tryptic activity is particularly low to limit damage to membrane proteins and receptors. Purified collagenase contains the least amount of secondary protein hydrolyzing activity as well as high collagenase activity. Levels of secondary proteases are similar to those of collagenase types 1 and 2. Purified collagenase contains minimal secondary protein hydrolyzing activity and high collagenase activity. Animal Free AFA (Collagenase (Animal Free) - Type A), AFB (Animal Free) - Type B) and AFC (Animal Free) - Type C), STZ1 and STZ2 collagenases are derived from cultures grown in media completely free of animal components, and are specifically designed for use in cultures where it is necessary to prevent the introduction of potential animal-derived pathogens.

The collagenases are derived from cultures grown in a completely animal-free medium and are designed for bioprocessing applications where the introduction of potential animal-derived pathogens must be prevented. Levels of secondary proteases are similar to those of collagenase types 1 and 2.CLSAFA is the original AFA grade and is intended to have collagenase and secondary proteases similar to those of collagenase types 1 and 2.CLSAFB contains a higher level of collagenase and caspase activity than CLSAFA.CLSAFC has exceptionally low trypsin activity similar to collagenase type 4.STZ1 and STZ2. 0.22μ filtered STEMxyme AOF collagenase/neutral protease (Dispase) mixture for primary and stem cell isolation.

Brand: MSE PRO™ 

Manufacturer: MSE Supplies LLC