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MSE PRO Ribonuclease T1, Chromatographically Purif., Lyophilized– MSE Supplies LLC

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MSE PRO Ribonuclease T1, Chromatographically Purif., Lyophilized

MSE PRO Ribonuclease T1, Chromatographically Purif., Lyophilized

SKU: BO0034

  • £41700
  • Save £4600

MSE PRO™ Ribonuclease T1, Chromatographically Purif., Lyophilized

The MSE PRO™ Ribonuclease T1 is an endonuclease with high specificity for cleaving RNA or deamidated RNA between a 3'-phosphoguanosine residue (or 3'-phosphoinositide) and the 5'-OH residue of an adjacent nucleotide and forming the corresponding 2', 3'-cyclic phosphate intermediate. It cleaves single-stranded RNA and releases oligonucleotides from the 3'-phosphoguanosine terminus. The molecular weight of the enzyme is 11 kDa. The optimum pH is 7.5. The stimulatory effect of histidine and ethylenediaminetetraacetic acid is due to chelation of contaminating inhibitory cations. The enzyme assay is essentially the method of Egami et al, Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964), which is based on the release of acid-soluble oligonucleotides after yeast RNA digestion.

Brand: MSE PRO™ 

Manufacturer: MSE Supplies LLC

MSE Supplies offers a variety of Biochemical Reagents. Please contact us for SDS, lead time and more information.

Specifications:

SKU# BO0034
EC No. 9026-12-4
EINECS No. 232-809-1
Uses Ribonuclease T1 has extensive applications in molecular cloning and DNA sequencing. Because of its specificity it has been a commonly used cleavage enzyme for the determination of structure, nearest neighbor frequencies, and RNA sequencing. The enzyme has further application in the preparation of nucleoside 2' ,3'-cyclic phosphates, the synthesis of oligonucleotides, and the removal of RNA from DNA preparations. The enzyme is also used as a non-mammalian source of RNase in various applications.
Stability Stable 12-18 months at 2-8°C
Technical Notes Some suppliers reference sequencing units; One sequencing unit is equivalent to 0.075 our unit.
Source Aspergillus oryzae
Description Highly purified, microbial (non-mammalian) broad specificity RNase prepared with non-animal components, supplied a as a lyophilized powder.
Storage Store at 2-8°C
Activity ≥2,500 units per mg dry weight
Unit Definition One Unit releases the equivalent of one A260 unit of acid-soluble products from yeast RNA in 15 minutes at 37°C, pH 7.5
Note Product packaging is subject to change, the picture is for reference only.