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Plant Flavonoids Colorimetric Assay Kit– MSE Supplies LLC

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Plant Flavonoids Colorimetric Assay Kit - MSE Supplies LLC

Plant Flavonoids Colorimetric Assay Kit

SKU: E-BC-K284-M-500

  • £50600
  • Save £8900

Plant Flavonoids Colorimetric Assay Kit

SKU # E-BC-K284-M
Detection Instrument Microplate reader (500 nm-520 nm, optimum wavelength: 510 nm)
Detection method Colorimetric method


Product Details

Properties

Sample type Plant tissue
Sensitivity 0.66 μg/mL
Detection range 0.66-150 μg/mL
Detection Method Colorimetric method
Assay type Quantitative
Assay time 35 min
Precision Average inter-assay CV: 5.300% | Average intra-assay CV: 4%
Other instruments required Micropipettor, Vortex mixer, Centrifuge, Vacuum dryer, Ultrasonic cell disruptor.
Other reagents required 60% alcohol
Storage 2-8℃
Valid period 12 months


Images

JH Kim et al investigate the bisphenol A-induced inflammation and cytotoxicity blocked by Grifola frondosa extract. Plant flavonoids level in plant tissue was determined using plant flavonoids colorimetric assay kit (E-BC-K284-M).

The concentration of total flavonoid was determined.

 

Dilution of Sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.66-150 μg/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Green dill 10-15
Green pepper 1
Pumpkin 1
Heather 25-35

 

Note: The diluent is 60% alcohol.

 

Detection Principle

In alkaline nitrite solution, flavonoids form red complex with aluminum ion. The flavonoid content of the sample can be calculated by measuring the absorbance of the sample extract at 510 nm.


Kit Components & Storage

Item Component Size 1 (48 T) Size 2 (96 T) Storage
Reagent 1 1 mg/mL Standard 1.8 mL × 1 vial 1.8 mL × 1 vial 2-8°C, 12 months
Reagent 2 Saline Solution 1 mL × 1 vial 1 mL × 2 vials 2-8°C, 12 months
Reagent 3 Aluminium Reagent 1.8 mL × 1 vial 1.8 mL × 2 vials 2-8°C, 12 months
Reagent 4 Alkali Reagent 15 mL × 1 vial 30 mL × 1 vial 2-8℃, 12 months
Microplate 96 wells No requirement
Plate Sealer 2 pieces

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)

Parameters Sample 1 Sample 2 Sample 3
Mean (μg/mL) 2.60 34.50 85.40
%CV 4.5 4.2 3.3


Inter-assay Precision

Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (μg/mL) 2.60 34.50 85.40
%CV 5.6 4.8 5.5


Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 103%.

Parameters Standard 1 Standard 2 Standard 3
Expected Conc. (μg/mL) 26 75 116
Observed Conc. (μg/mL) 27.3 77.3 117.2
Recovery rate (%) 105 103 101


Sensitivity

The analytical sensitivity of the assay is 0.66 μg/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.


Standard Curve

As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:

Concentration (μg/mL) 0 20 40 60 80 100 120 150
OD value 0.041 0.082 0.132 0.181 0.235 0.272 0.338 0.405
0.040 0.083 0.134 0.183 0.233 0.281 0.340 0.413
Average OD 0.041 0.082 0.133 0.182 0.234 0.277 0.339 0.409
Absoluted OD 0.000 0.042 0.093 0.142 0.194 0.237 0.299 0.369