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Polyphenol Oxidase (PPO) Activity Assay Kit
SKU: E-BC-K259-M-96
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Polyphenol Oxidase (PPO) Activity Assay Kit
| SKU # | E-BC-K259-M |
| Detection Instrument | Microplate reader (405 nm-415 nm, optimum wavelength: 410 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | PPO |
| Sample type | Plant tissue |
| Detection Method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 25 min |
| Precision | Average inter-assay CV: 8.700% | Average intra-assay CV: 4.600% |
| Other instruments required | Tubes, Micropipette, Vortex mixer, 100℃ Water bath |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| 10% Ginger tissue homogenization | 1 |
| 10% Chinese yam tissue homogenization | 1 |
| 10% Corn tissue homogenization | 1 |
| 10% Pear tissue homogenization | 1 |
Note: For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
Polyphenol oxidase (PPO) can catalyze phenolic compounds into quinone substances. The latter has specific absorption at 410 nm. The activity of PPO can be calculated indirectly bymeasuring the OD value at 410 nm.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Extracting Solution | 60 mL × 1 vial | 60 mL × 2 vials | 2-8°C, 12 months |
| Reagent 2 | Buffer Solution | 40 mL × 1 vial | 40 mL × 2 vials | 2-8°C, 12 months |
| Reagent 3 | Substrate | 10 mL × 1 vial | 20 mL × 1 vial | 2-8°C, 12 months shading light |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.