Rat IL-10 (Interleukin 10) CLIA Kit
SKU: E-CL-R0016
Rat IL-10 (Interleukin 10) CLIA Kit
Detection Range | 7.81-500 pg/mL |
Sensitivity | 4.69 pg/mL |
Product Details
Properties
Assay type | Sandwich-CLIA |
Format | 96T |
Assay time | 3.5h |
Detection range | 7.81-500 pg/mL |
Sensitivity | 4.69 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat IL-10 in samples. No significant cross-reactivity or interference between Rat IL-10 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 15%. |
Application | This CLIA kit applies to the in vitro quantitative determination of Rat IL-10 concentrations in serum, plasma and other biological fluids. |
Test Principle
Kit components & Storage
Item | Specifications | Storage |
---|---|---|
Micro CLIA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips |
-20℃, 12 months |
Reference Standard | 96T: 2 vials 48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 48T: 1 vial, 60 μL |
-20℃(Protect from light), 12 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 12 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃(Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Technical Data
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IL-10 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IL-10 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 27.08 | 77.45 | 249.63 | 24.70 | 75.89 | 241.12 |
Standard deviation | 3.06 | 6.53 | 16.38 | 3.18 | 8.33 | 19.94 |
CV (%) | 11.30 | 8.43 | 6.56 | 12.87 | 10.98 | 8.27 |
Recovery
The recovery of Rat IL-10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 99-112 | 104 |
EDTA plasma(n=8) | 91-104 | 96 |
Cell culture media(n=8) | 97-113 | 103 |
Target Information
Synonyms | IL10, IL10A, CSIF, TGIF |
Research Area | Cancer, Immunology |
Assay Procedures
1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃. |
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2.Remove the liquid. |
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3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times. |
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4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times. |
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5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃. |
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6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results. |