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Recombinant Human CAMK2A/CAMKA Protein (GST Tag)(Active)
SKU: PKSH030403-50
Recombinant Human CAMK2A/CAMKA Protein (GST Tag)(Active)
| SKU # | PKSH030403 |
| Expression Host | Baculovirus-Insect Cells |
Description
| Synonyms | CAMKA |
| Species | Human |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met 1-His 478 |
| Accession | NP_741960.1 |
| Calculated Molecular Weight | 80.3 kDa |
| Observed Molecular Weight | 80 kDa |
| Tag | N-GST |
| Bio-activity | The specific activity was determined to be 160 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate. |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as sterile solution of 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM Reduced Glutathione, pH 8.0 |
| Reconstitution | Not Applicable |
Background
Ca2+/calmodulin-dependent protein kinase2A (CAMK2A) belongs to the serine/threonine protein kinase family and, together with other 28 different isoforms, belongs to the Ca2+/ calmodulin-dependent protein kinase subfamily. CaM kinase Ⅱ is thought to be an important mediator of learning and memory and is also necessary for Ca2+ homeostasis and reuptake in cardiomyocytes chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CAMKIIA is one of the major forms of CAMKII. It has been found to play a critical role in sustaining activation of CAMKII at the postsynaptic density. Studies have found that knockout mice without CAMKIIA demonstrate a low frequency of LTP. Additionally, these mice do not form persistent, stable place cells in the hippocampus.