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Recombinant Human EphA3 Protein (His Tag)(Active)
SKU: PKSH031116-100
Recombinant Human EphA3 Protein (His Tag)(Active)
| SKU # | PKSH031116 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | EK4, ETK, ETK1, HEK, HEK4, TYRO4 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Gln541 |
| Accession | NP_005224.2 |
| Calculated Molecular Weight | 60.3 kDa |
| Observed Molecular Weight | 65-70 kDa |
| Tag | C-His |
| Bio-activity | Immobilized human EPHA3-His at 10 μg/ml (100 μl/well) can bind human EphrinA5-Fc, The EC50 of human EphrinA5-Fc is 6. 2-14.6 ng/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.