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Recombinant Human HER2/ErbB2 Protein (His Tag)(Active)
SKU: PKSH031997-100
Recombinant Human HER2/ErbB2 Protein (His Tag)(Active)
| SKU # | PKSH031997 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD340, ENV, ENVW, ERBB2, ERVWE1, HER-2, HER-2/neu, HER2, HERV-7q, HERV-W-ENV, HERV7Q, HERVW, HERVWENV, MLN 1, MLN19, Metastatic lymph node gene 19 protein, NGL, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Thr 652 |
| Accession | NP_004439.2 |
| Calculated Molecular Weight | 71.0 kDa |
| Observed Molecular Weight | 100-110 kDa |
| Tag | C-His |
| Bio-activity | Immobilized human Erbb2 at 2. 5 μg/ml (100 μl/well) can bind Herceptin with a linear ranger of 1. 28-32 ng/ml. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.