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Recombinant Human IDH1 Protein (E.coli, His Tag)
SKU: PKSH030920-50
Recombinant Human IDH1 Protein (E.coli, His Tag)
| SKU # | PKSH030920 |
| Expression Host | E.coli |
Description
| Synonyms | Cytosolic NADP-Isocitrate Dehydrogenase, IDH, IDH1, IDP, Isocitrate Dehydrogenase [NADP] Cytoplasmic, NADP(+)-Specific ICDH, Oxalosuccinate Decarboxylase, PICD |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Leu 414 |
| Accession | O75874 |
| Calculated Molecular Weight | 48.0 kDa |
| Observed Molecular Weight | 43 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 50mM Tris, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer; binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions; such as the conversion of 2; 4-dienoyl-CoAs to 3-enoyl-CoAs; as well as in peroxisomal reactions that consume 2-oxoglutarate; namely the α-hydroxylation of phytanic acid.