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Recombinant Human PCNA protein (His tag)
SKU: PDEH100277-100
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Recombinant Human PCNA protein (His tag)
| SKU # | PDEH100277 |
| Expression Host | E. coli |
Description
| Synonyms | ATLD2;cb16;Cyclin;DNA polymerase delta auxiliary protein;Mutagen-sensitive 209 protein;Polymerase delta accessory protein;Proliferating cell nuclear antigen |
| Species | Human |
| Expression_host | E.coli |
| Sequence | Met 1-Ser 261 |
| Accession | P12004 |
| Mol_Mass | 28.6 kDa |
| AP_Mol_Mass | 38 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.