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Recombinant Human PDGF-BB Protein (Active)
SKU: PKSH032906-50
Recombinant Human PDGF-BB Protein (Active)
| SKU # | PKSH032906 |
| Expression Host | E.coli |
Description
| Synonyms | Becaplermin, PDGF Subunit B, PDGF-2, PDGF2, PDGFB, Platelet-Derived Growth Factor B Chain, Platelet-Derived Growth Factor Beta Polypeptide, Platelet-Derived Growth Factor Subunit B, Proto-Oncogene c-Sis, SIS |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ser82-Thr190 |
| Accession | P01127 |
| Calculated Molecular Weight | 12.4 kDa |
| Observed Molecular Weight | 14 kDa |
| Tag | None |
| Bio-activity | Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 5-20 ng/ml. |
Properties
| Purity | > 98 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 0.2 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM NaAc-HAc, pH 4.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.