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Recombinant Human PDGF-BB Protein (His Tag)(Active)
SKU: PKSH030442-50
Recombinant Human PDGF-BB Protein (His Tag)(Active)
| SKU # | PKSH030442 |
| Expression Host | Yeast |
Description
| Synonyms | Becaplermin, PDGF Subunit B, PDGF-2, PDGF2, PDGFB, Platelet-Derived Growth Factor B Chain, Platelet-Derived Growth Factor Beta Polypeptide, Platelet-Derived Growth Factor Subunit B, Proto-Oncogene c-Sis, SIS |
| Species | Human |
| Expression Host | Yeast |
| Sequence | Ser82-Thr190 |
| Accession | NP_148937.1 |
| Calculated Molecular Weight | 14.3 kDa |
| Tag | N-His |
| Bio-activity | Immobilized human PDGFB at 10μg/mL (100μL/well) can bind human PDGFRβ-Fch, the EC50 of human PDGFRβ-Fch is 0.05-0.2 μg/mL. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, 25 % Glycerol, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Platelet-Derived Growth Factor Subunit B (PDGFB) belongs to the PDGF/VEGF growth factor family. Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.Binding of PDGFB to its receptor elicits a variety of cellular responses. In addition, PDGFB is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound.