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Recombinant Human UBE2A Protein (His Tag)
SKU: PKSH030787-100
Recombinant Human UBE2A Protein (His Tag)
| SKU # | PKSH030787 |
| Expression Host | E.coli |
Description
| Synonyms | HR6A, RAD6 Homolog A, RAD6A, UBE2A, Ubiquitin Carrier Protein A, Ubiquitin-Conjugating Enzyme E2 A, Ubiquitin-Protein Ligase A, hHR6A |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Cys 152 |
| Accession | P49459 |
| Calculated Molecular Weight | 19.2 kDa |
| Observed Molecular Weight | 18.5 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, 20% glycerol, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Ubiquitin-conjugating enzyme E2 A (also known as HHR6A or UBE2A); encoded by human DNA repair genes HHR6A; belongs to the ubiquitin-conjugating enzymes (E2 enzymes) family and is likely to be involved in postreplication repair and induced mutagenesis. UBE2A is described as a CDK2 substrate. It is the human homologue of the product of the Saccharomyces cerevisiae RAD6 / UBC2 gene; a member of the family of ubiquitin-conjugating enzymes. In vivo; HHR6A phosphorylation peaks during the G2/M phase of cell cycle transition; with a concomitant increase in histone H2B ubiquitylation. Mutation of Ser120 to threonine or alanine abolished UBE2A activity; while mutation to aspartate to mimic phosphorylated serine increased UBE2A activity 3-fold. A mutation of UBE2A is consisdered as the cause of a novel X-linked mental retardation (XLMR) syndrome that affects three males in a two-generation family.