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Recombinant Rat TNF-αsf protein(His tag)
SKU: PDER100019-100
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Recombinant Rat TNF-αsf protein(His tag)
| SKU # | PDER100019 |
| Expression Host | E. coli |
Description
| Synonyms | Cachectin;TNF-alpha;Tumor necrosis factor ligand superfamily member 2;TNF-a;N-terminal fragment;NTF;ICD1;ICD2 |
| Species | Rat |
| Expression_host | E.coli |
| Sequence | Gly57-Leu235 |
| Accession | P16599 |
| Mol_Mass | 19.7 kDa |
| AP_Mol_Mass | 19 kDa |
| Tag | N-His |
| Bio_Activity | Not validated for activity |
Properties
| Purity | > 85 % as determined by reducing SDS-PAGE. |
| Endotoxin level | Please contact us for more information. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis |
Background
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance.Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.